Coding
Str(e)pBC

Part:BBa_K4590004:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


ABC Transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 431
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1120
    Illegal NgoMIV site found at 1270
    Illegal AgeI site found at 1307
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1261


Design Notes

The membrane transporter chosen for overexpression is part of the cosmomycin cluster.


Source

Streptomyces olindensis strain DAUFPE 5622 2, whole genome shotgun sequence GenBank: JJOH01000002.1

References